Hotspot

ndhC-trnV-UAC

LSC · 54342-55687 · 1346 bp

Back to Hibiscus

Pi 0.0461

MarkerSeek score 82.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1346
variable_sites: 152
pi: 0.0461
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ACCCTTTCTAATCGGGGCTA	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.8	58.7	1329-1581 bp	1.0	85.0
ndhC-trnV-UAC_p2	CGGGGCTAAAACTCCAGAAA	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.8	58.7	1317-1569 bp	1.0	85.0
ndhC-trnV-UAC_p3	ATCGGGGCTAAAACTCCAGA	GTTCGAGTCCGTATAGCCCT	50.0	55.0	58.4	58.7	1319-1571 bp	1.0	83.2
ndhC-trnV-UAC_p4	AATCGGGGCTAAAACTCCAG	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.6	58.7	1320-1572 bp	1.0	83.2
ndhC-trnV-UAC_p5	ACCCTTTCTAATCGGGGCTA	TTCGAGTCCGTATAGCCCTA	50.0	50.0	57.8	57.1	1328-1580 bp	1.0	83.0