Hotspot

rps4-trnT-UGU

LSC · 49003-49643 · 641 bp

Back to Hibiscus

Pi 0.0656

MarkerSeek score 79.30

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 641
variable_sites: 102
pi: 0.0656
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	GCTTTTCTGGCAATCCGAAC	GGTGGAATACTCGAACGGTC	50.0	55.0	58.0	58.1	1404-1662 bp	1.0	91.2
rps4-trnT-UGU_p2	TAAGATCGCTTCCGGCTCTA	GGTGGAATACTCGAACGGTC	50.0	55.0	58.0	58.1	1277-1535 bp	1.0	91.2
rps4-trnT-UGU_p3	CGAAGCCATACCCAATCGAA	GGTGGAATACTCGAACGGTC	50.0	55.0	58.1	58.1	1486-1744 bp	1.0	90.9
rps4-trnT-UGU_p4	TTTCTAAGATCGCTTCCGGC	GGTGGAATACTCGAACGGTC	50.0	55.0	58.1	58.1	1281-1539 bp	1.0	90.8
rps4-trnT-UGU_p5	AAAACCTGACCTGTTGACCC	GGTGGAATACTCGAACGGTC	50.0	55.0	57.9	58.1	1428-1686 bp	1.0	90.8