Hotspot

psbK-psbI

LSC · 7700-8138 · 439 bp

Back to Hibiscus

Pi 0.0343

MarkerSeek score 78.80

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 439
variable_sites: 41
pi: 0.0343
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	GGATTACGTCCCGGATCATT	50.0	50.0	57.9	57.2	559-581 bp	1.0	81.7
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TTTTTATTCTTCGCGCCCAG	50.0	45.0	57.9	57.1	578-600 bp	1.0	80.9
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	TTTTATTCTTCGCGCCCAGG	50.0	50.0	57.9	58.9	577-599 bp	1.0	80.8
psbK-psbI_p4	TGGCAAGCTGCTGTAAGTTT	GGATTACGTCCCGGATCATT	45.0	50.0	58.3	57.2	556-578 bp	1.0	79.7
psbK-psbI_p5	TTGGCAAGCTGCTGTAAGTT	GGATTACGTCCCGGATCATT	45.0	50.0	58.3	57.2	557-579 bp	1.0	79.7