Hotspot

ndhF-rpl32

SSC · 118190-119180 · 991 bp

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Pi 0.0107
MarkerSeek score 58.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
991
variable_sites
31
pi
0.0107
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TTTGTTGCTGCCGGAAAAAG CGCTAACGAAAAAGCTTTCA 45.0 40.0 58.1 55.2 1197-1200 bp 1.0 59.5
ndhF-rpl32_p2 GGGACTGGCAATGTAACGAA CGCTAACGAAAAAGCTTTCA 50.0 40.0 58.2 55.2 1152-1155 bp 1.0 58.7
ndhF-rpl32_p3 AAAAAGCAGGAGTCCCACTC CGCTAACGAAAAAGCTTTCA 50.0 40.0 57.7 55.2 1183-1186 bp 1.0 57.8
ndhF-rpl32_p4 GAAAAAGCAGGAGTCCCACT CGCTAACGAAAAAGCTTTCA 50.0 40.0 57.7 55.2 1184-1187 bp 1.0 57.8
ndhF-rpl32_p5 TTTGTTGCTGCCGGAAAAAG CGCTAACGAAAAAGCTTTCAA 45.0 38.1 58.1 56.0 1197-1200 bp 1.0 57.7