Hotspot

rpl22-rps19

LSC · 88852-88928 · 77 bp

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Pi 0.013
MarkerSeek score 47.60
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
77
variable_sites
3
pi
0.013
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 TTCTTCGTGCTTTGTGAGGG TGGTGGGTCATAAATTGGGAG 50.0 47.6 58.4 57.6 254 bp 1.0 66.6
rpl22-rps19_p2 GCTTTGTGAGGGGACATACA TGGTGGGTCATAAATTGGGAG 50.0 47.6 57.5 57.6 246 bp 1.0 66.0
rpl22-rps19_p3 GTGCTTTGTGAGGGGACATA TGGTGGGTCATAAATTGGGAG 50.0 47.6 57.5 57.6 248 bp 1.0 66.0
rpl22-rps19_p4 TGCTTTGTGAGGGGACATAC TGGTGGGTCATAAATTGGGAG 50.0 47.6 57.5 57.6 247 bp 1.0 66.0
rpl22-rps19_p5 TTCTTCGTGCTTTGTGAGGG TGGTGGGTCATAAATTGGGA 50.0 45.0 58.4 56.4 254 bp 1.0 64.8