Hotspot

ndhC-trnC(ACA)

LSC · 53679-54337 · 659 bp

Back to Hevea

Pi 0.00334
MarkerSeek score 49.30
Primer available yes
Label ndhC-trnC(ACA)

Feature Metadata

label_name
ndhC-trnC(ACA)
feature_type
igs
region
LSC
length_bp
659
variable_sites
6
pi
0.00334
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnC(ACA)_p1 CCCTTTGCTAATCGGGGATA AGAAGGTCTACGGTTCGAGT 50.0 50.0 56.8 57.8 803-838 bp 1.0 69.3
ndhC-trnC(ACA)_p2 CTCCGGCCCTTTGCTAATC AGAAGGTCTACGGTTCGAGT 57.9 50.0 58.3 57.8 809-844 bp 1.0 68.9
ndhC-trnC(ACA)_p3 TTCTCCGGCCCTTTGCTAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 59.4 57.8 811-846 bp 1.0 68.2
ndhC-trnC(ACA)_p4 CCCTTTGCTAATCGGGGATAA AGAAGGTCTACGGTTCGAGT 47.6 50.0 57.5 57.8 803-838 bp 1.0 67.2
ndhC-trnC(ACA)_p5 CCCTTTGCTAATCGGGGATA GAAGGTCTACGGTTCGAGTC 50.0 55.0 56.8 57.5 802-837 bp 1.0 66.5