Hotspot

trnR(TCT)-trnT(GGT)

LSC · 31941-34410 · 2470 bp

Back to Hevea

Pi 0.00108
MarkerSeek score 48.60
Primer available yes
Label trnR(TCT)-trnT(GGT)

Feature Metadata

label_name
trnR(TCT)-trnT(GGT)
feature_type
igs
region
LSC
length_bp
2470
variable_sites
7
pi
0.00108
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR(TCT)-trnT(GGT)_p1 AGAAGACCTCTGTCCTATCCA GAACCGATGACTTACGCCTT 47.6 50.0 56.9 58.0 2561-2701 bp 1.0 64.0
trnR(TCT)-trnT(GGT)_p2 AGGTTTAGAAGACCTCTGTCCT GAACCGATGACTTACGCCTT 45.5 50.0 57.9 58.0 2567-2707 bp 1.0 63.3
trnR(TCT)-trnT(GGT)_p3 AGAAGACCTCTGTCCTATCCA TACCGCTGAGTTAAAAGGGC 47.6 50.0 56.9 57.9 2526-2666 bp 1.0 63.2
trnR(TCT)-trnT(GGT)_p4 AGAAGACCTCTGTCCTATCCA CCGATGACTTACGCCTTACC 47.6 55.0 56.9 58.2 2558-2698 bp 1.0 62.8
trnR(TCT)-trnT(GGT)_p5 AGGTTTAGAAGACCTCTGTCCT TACCGCTGAGTTAAAAGGGC 45.5 50.0 57.9 57.9 2532-2672 bp 1.0 62.6