Hotspot

atpH-atpF

LSC · 28977-29581 · 605 bp

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Pi 0.00176
MarkerSeek score 49.00
Primer available yes
Label atpH-atpF

Feature Metadata

label_name
atpH-atpF
feature_type
igs
region
LSC
length_bp
605
variable_sites
2
pi
0.00176
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpF_p1 CGGAGGGAAAAATACGAGGT CAGTGACCCAAGGAAACGAA 50.0 50.0 57.3 58.0 752-760 bp 1.0 75.3
atpH-atpF_p2 CGAGGCGGAGGGAAAAATAC CAGTGACCCAAGGAAACGAA 55.0 50.0 58.7 58.0 757-765 bp 1.0 75.1
atpH-atpF_p3 GAGGCGGAGGGAAAAATACG CAGTGACCCAAGGAAACGAA 55.0 50.0 58.7 58.0 756-764 bp 1.0 75.1
atpH-atpF_p4 GCGGAGGGAAAAATACGAGG CAGTGACCCAAGGAAACGAA 55.0 50.0 58.7 58.0 753-761 bp 1.0 75.1
atpH-atpF_p5 GGCGGAGGGAAAAATACGAG CAGTGACCCAAGGAAACGAA 55.0 50.0 58.7 58.0 754-762 bp 1.0 75.1