Hotspot

psbM-petN

LSC · 11835-13199 · 1365 bp

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Pi 0.000781
MarkerSeek score 47.10
Primer available yes
Label psbM-petN

Feature Metadata

label_name
psbM-petN
feature_type
igs
region
LSC
length_bp
1365
variable_sites
2
pi
0.000781
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-petN_p1 GCGACTGCACTGTTCATTCT TAGTAAGTCTCGCTTGGGCT 50.0 50.0 58.8 58.2 1522-1526 bp 1.0 72.3
psbM-petN_p2 GCGACTGCACTGTTCATTCT ATGGGGAAGAAGTGGACTCT 50.0 50.0 58.8 57.6 1461-1465 bp 1.0 70.7
psbM-petN_p3 GCGACTGCACTGTTCATTCT ATAGTAAGTCTCGCTTGGGC 50.0 50.0 58.8 57.1 1523-1527 bp 1.0 66.4
psbM-petN_p4 GCGACTGCACTGTTCATTCT CTCGCTTGGGCTGCTTTAAT 50.0 50.0 58.8 58.9 1514-1518 bp 1.0 66.3
psbM-petN_p5 GCGACTGCACTGTTCATTCTA TAGTAAGTCTCGCTTGGGCT 47.6 50.0 58.7 58.2 1522-1526 bp 1.0 65.8