Hotspot

ndhF

SSC · 111761-114004 · 2244 bp

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Pi 0.006

MarkerSeek score 40.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2244
variable_sites: 39
pi: 0.006
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 40.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAACGGAAAACCCTTCCTC	ACGGATATCATAGCGTCTCCT	50.0	47.6	58.0	57.9	2901-2904 bp	0.166667	38.0
ndhF_p2	CAACGGAAAACCCTTCCTCT	ACGGATATCATAGCGTCTCCT	50.0	47.6	57.7	57.9	2900-2903 bp	0.166667	36.0
ndhF_p3	CACAACGGAAAACCCTTCCT	ACGGATATCATAGCGTCTCCT	50.0	47.6	58.3	57.9	2902-2905 bp	0.166667	35.7
ndhF_p4	ACAACGGAAAACCCTTCCTC	GACGGATATCATAGCGTCTCC	50.0	52.4	58.0	57.6	2902-2905 bp	0.166667	35.3
ndhF_p5	ACAACGGAAAACCCTTCCTC	ACGGATATCATAGCGTCTCC	50.0	50.0	58.0	56.4	2901-2904 bp	0.166667	33.8