Hotspot

ndhD

SSC · 117125-118627 · 1503 bp

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Pi 0.00678

MarkerSeek score 38.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 27
pi: 0.00678
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GTAAGCCCACGAGAACCATT	TCCACGCGAATCCATTTGAT	50.0	45.0	57.9	58.0	1777-1791 bp	1.0	81.0
ndhD_p2	GCTAATGAGGCATGGGGATC	TCCACGCGAATCCATTTGAT	55.0	45.0	58.2	58.0	2069-2080 bp	1.0	80.7
ndhD_p3	TCAGGAGCAGTATGGGCTAA	TCCACGCGAATCCATTTGAT	50.0	45.0	57.8	58.0	2084-2095 bp	1.0	80.6
ndhD_p4	TTCAGGAGCAGTATGGGCTA	TCCACGCGAATCCATTTGAT	50.0	45.0	57.8	58.0	2085-2096 bp	1.0	80.6
ndhD_p5	GTAAGCCCACGAGAACCATT	TGCTTCTGCTCCAAGAACAG	50.0	50.0	57.9	58.1	1922-1936 bp	1.0	80.3