Hotspot

ndhC-trnV-UAC

LSC · 52320-53411 · 1092 bp

Back to Heuchera

Pi 0.00876
MarkerSeek score 66.30
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1092
variable_sites
28
pi
0.00876
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GTCCTTTGCTAATCGGAGCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1227-1259 bp 1.0 80.7
ndhC-trnV-UAC_p2 GTCCTTTGCTAATCGGAGCT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.7 57.5 1226-1258 bp 1.0 78.0
ndhC-trnV-UAC_p3 CTGGTCCTTTGCTAATCGGA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 1230-1262 bp 1.0 77.7
ndhC-trnV-UAC_p4 TCTGGTCCTTTGCTAATCGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 1231-1263 bp 1.0 77.7
ndhC-trnV-UAC_p5 TGGTCCTTTGCTAATCGGAG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 1229-1261 bp 1.0 77.7