Hotspot

trnT-trnL-UAA

LSC · 48153-49137 · 985 bp

Back to Heuchera

Pi 0.0141

MarkerSeek score 55.00

Primer available yes

Label trnT-trnL-UAA

Feature Metadata

label_name: trnT-trnL-UAA
feature_type: igs
region: LSC
length_bp: 985
variable_sites: 34
pi: 0.0141
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 55.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	930-1042 bp	1.0	85.2
trnT-trnL-UAA_p2	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	962-1074 bp	1.0	84.3
trnT-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	936-1048 bp	1.0	83.8
trnT-trnL-UAA_p4	GGAATCGAACCGATGACCAT	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	968-1080 bp	1.0	82.9
trnT-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	TAAGTCCGTAGCGTCTACCA	55.0	50.0	57.9	57.6	938-1050 bp	1.0	82.5