Hotspot

psbZ-trnG-UCC

LSC · 37782-38259 · 478 bp

Back to Heuchera

Pi 0.0312

MarkerSeek score 75.90

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 478
variable_sites: 45
pi: 0.0312
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TTTGCTTCTCCTGACGGTTG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.4	57.8	605-879 bp	1.0	83.9
psbZ-trnG-UCC_p2	ATTTGCTTCTCCTGACGGTT	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.4	57.8	606-880 bp	1.0	82.6
psbZ-trnG-UCC_p3	GTATTTGCTTCTCCTGACGGT	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.0	57.8	608-882 bp	1.0	78.9
psbZ-trnG-UCC_p4	TGTATTTGCTTCTCCTGACGG	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.0	57.8	609-883 bp	1.0	78.9
psbZ-trnG-UCC_p5	CCCGTTGTATTTGCTTCTCC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.8	57.8	614-888 bp	1.0	77.4