Hotspot

rpoB-trnC-GCA

LSC · 27634-28855 · 1222 bp

Back to Heuchera

Pi 0.00682

MarkerSeek score 54.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1222
variable_sites: 26
pi: 0.00682
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AAAGTTCTTCCGTCAAGCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.4	58.1	1386-1404 bp	1.0	78.4
rpoB-trnC-GCA_p2	TCCCTCATTTCCATTCCGGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.4	58.1	1304-1322 bp	1.0	78.4
rpoB-trnC-GCA_p3	TTCCCTCATTTCCATTCCGG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.6	58.1	1305-1323 bp	1.0	78.2
rpoB-trnC-GCA_p4	CCGTCAAGCCCTGATCAATA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.4	58.1	1377-1395 bp	1.0	76.6
rpoB-trnC-GCA_p5	AAAGTTCTTCCGTCAAGCCC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.4	58.4	1368-1386 bp	1.0	76.3