Hotspot

trnR-UCU-atpA

LSC · 10602-10817 · 216 bp

Back to Heuchera

Pi 0.0255

MarkerSeek score 56.90

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 216
variable_sites: 15
pi: 0.0255
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	ATGAAAGGCGTCCATTGTCT	GAGGAAGCAGAAGCCCTTTT	45.0	50.0	57.5	58.1	343-370 bp	1.0	82.9
trnR-UCU-atpA_p2	GGAATGAAAGGCGTCCATTG	GAGGAAGCAGAAGCCCTTTT	50.0	50.0	57.4	58.1	346-373 bp	1.0	82.3
trnR-UCU-atpA_p3	ATGAAAGGCGTCCATTGTCT	AGGAAGCAGAAGCCCTTTTG	45.0	50.0	57.5	58.4	342-369 bp	0.916667	77.0
trnR-UCU-atpA_p4	ATGAAAGGCGTCCATTGTCT	GGAAGCAGAAGCCCTTTTGA	45.0	50.0	57.5	58.4	341-368 bp	0.916667	77.0
trnR-UCU-atpA_p5	GGAATGAAAGGCGTCCATTG	AGGAAGCAGAAGCCCTTTTG	50.0	50.0	57.4	58.4	345-372 bp	0.916667	76.5