Hotspot

ycf2

IRb · 87549-94301 · 6753 bp

Back to Heteropolygonatum

Pi 0.00133

MarkerSeek score 54.40

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRb
length_bp: 6753
variable_sites: 16
pi: 0.00133
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TTCTATAGGAACTGGGCGGT	ATCGGTCTATTTTCGGCGTC	50.0	50.0	57.8	58.2	114-251 bp	1.0	84.0
ycf2_p2	TCTATAGGAACTGGGCGGTC	ATCGGTCTATTTTCGGCGTC	55.0	50.0	58.3	58.2	113-250 bp	1.0	83.0
ycf2_p3	TTCTATAGGAACTGGGCGGT	AATCGGTCTATTTTCGGCGT	50.0	45.0	57.8	57.7	113-252 bp	1.0	82.7
ycf2_p4	TTCTATAGGAACTGGGCGGT	CGGTCTATTTTCGGCGTCAT	50.0	50.0	57.8	58.4	113-249 bp	1.0	81.8
ycf2_p5	TCTATAGGAACTGGGCGGTC	AATCGGTCTATTTTCGGCGT	55.0	45.0	58.3	57.7	112-251 bp	1.0	81.7