Hotspot

ycf1

SSC · 125081-130519 · 5439 bp

Back to Heteropolygonatum

Pi 0.00474

MarkerSeek score 43.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5439
variable_sites: 58
pi: 0.00474
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGAAAAGGATAGGTGTGGTGA	CCATTTCCAACGCTGAAACA	42.9	45.0	56.3	57.2	114-256 bp	1.0	53.9
ycf1_p2	AAGAAAAGGATAGGTGTGGTGA	CCATTTCCAACGCTGAAACA	40.9	45.0	57.0	57.2	115-257 bp	1.0	51.8
ycf1_p3	AGAAAAGGATAGGTGTGGTGA	TCCATTTCCAACGCTGAAAC	42.9	45.0	56.3	56.9	114-257 bp	1.0	51.6
ycf1_p4	AGAAAAGGATAGGTGTGGTGA	CCATTTCCAACGCTGAAACAA	42.9	42.9	56.3	57.9	115-256 bp	1.0	51.5
ycf1_p5	AGAAAAGGATAGGTGTGGTGAT	CCATTTCCAACGCTGAAACA	40.9	45.0	56.8	57.2	114-256 bp	1.0	50.1