Hotspot

ndhG-ndhI

SSC · 120030-120409 · 380 bp

Back to Heteropolygonatum

Pi 0.0137

MarkerSeek score 42.70

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 380
variable_sites: 10
pi: 0.0137
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	ACTCCATCTCCAACAGTCCA	AGCAGCAAGGTACATTGGTC	50.0	50.0	58.0	58.2	115-1169 bp	1.0	79.6
ndhG-ndhI_p2	ACTCCATCTCCAACAGTCCA	GAGCAGCAAGGTACATTGGT	50.0	50.0	58.0	58.2	114-1170 bp	1.0	79.6
ndhG-ndhI_p3	ACTCCATCTCCAACAGTCCA	AACAATACGAGCAGCAAGGT	50.0	45.0	58.0	57.8	114-1178 bp	1.0	79.5
ndhG-ndhI_p4	ACTCCATCTCCAACAGTCCA	ACCCCCTTGATTCAAGAACG	50.0	50.0	58.0	57.8	115-711 bp	0.8	72.0
ndhG-ndhI_p5	ACTCCATCTCCAACAGTCCA	CCCCTTGATTCAAGAACGGT	50.0	50.0	58.0	57.8	114-709 bp	0.8	72.0