Hotspot

ndhF-rpl32

SSC · 113292-114282 · 991 bp

Back to Heteropolygonatum

Pi 0.00673

MarkerSeek score 56.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 991
variable_sites: 14
pi: 0.00673
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCTGTCGGAATAAGCA	ACTGACTTTGCTAAAGAAAAAGCT	45.0	33.3	57.8	57.5	116-1209 bp	1.0	44.7
ndhF-rpl32_p2	TGTTGCTGTCGGAATAAGCA	ACTGACTTTGCTAAAGAAAAAGC	45.0	34.8	57.8	56.2	116-1209 bp	1.0	42.0
ndhF-rpl32_p3	TGCTGTCGGAATAAGCAGAA	ACTGACTTTGCTAAAGAAAAAGCT	45.0	33.3	57.2	57.5	116-1206 bp	1.0	41.8
ndhF-rpl32_p4	TTGCTGTCGGAATAAGCAGA	ACTGACTTTGCTAAAGAAAAAGCT	45.0	33.3	57.2	57.5	117-1207 bp	1.0	41.8
ndhF-rpl32_p5	TGTTGCTGTCGGAATAAGCA	CTGACTTTGCTAAAGAAAAAGCT	45.0	34.8	57.8	55.9	115-1208 bp	1.0	41.8