Hotspot

petA-psbJ

LSC · 63941-64988 · 1048 bp

Back to Heteropolygonatum

Pi 0.00693

MarkerSeek score 63.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1048
variable_sites: 17
pi: 0.00693
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCCGTTATTTTGGCAC	CCGGATTGGGTTCATCTCTG	50.0	55.0	58.1	58.0	113-1156 bp	1.0	80.6
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	CCGGATTGGGTTCATCTCTG	50.0	55.0	57.8	58.0	113-1173 bp	1.0	79.9
petA-psbJ_p3	GGCATCCGTTATTTTGGCAC	TCCGGATTGGGTTCATCTCT	50.0	50.0	58.1	58.1	112-1157 bp	1.0	79.9
petA-psbJ_p4	GGCCTTTTGTTCTTCTTGGC	TCCGGATTGGGTTCATCTCT	50.0	50.0	57.8	58.1	112-1174 bp	1.0	79.3
petA-psbJ_p5	TGTTCTTCTTGGCATCCGTT	CCGGATTGGGTTCATCTCTG	45.0	55.0	57.7	58.0	113-1166 bp	1.0	79.0