Hotspot

trnC-GCA-petN

LSC · 28545-29426 · 882 bp

Back to Heteropolygonatum

Pi 0.00975

MarkerSeek score 56.30

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 882
variable_sites: 16
pi: 0.00975
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTA	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	57.5	58.0	963-966 bp	1.0	77.1
trnC-GCA-petN_p2	GGGGGACTGCAAATCCTTTA	AGCAATTAGTAGTGCCCAGC	50.0	50.0	57.5	58.0	1040-1043 bp	1.0	76.7
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTA	AGTCCACTCCTTCCCCATAC	50.0	55.0	57.5	58.1	1015-1018 bp	1.0	76.1
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTA	GTCCACTCCTTCCCCATACT	50.0	55.0	57.5	58.1	1014-1017 bp	1.0	76.1
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTA	GAGTCCACTCCTTCCCCATA	50.0	55.0	57.5	57.8	1016-1019 bp	1.0	75.6