Hotspot

rpoC2

LSC · 25793-30307 · 4515 bp

Back to Heteropogon

Pi 0.00532

MarkerSeek score 43.80

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4515
variable_sites: 54
pi: 0.00532
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	ATAGGACCCTAGAGGAAGAA	ATATTCGCCCTCTCCCTCTC	45.0	55.0	53.6	58.1	125-242 bp	1.0	50.8
rpoC2_p2	ATAGGACCCTAGAGGAAGAA	TTCATATTCGCCCTCTCCCT	45.0	50.0	53.6	57.9	128-245 bp	1.0	50.4
rpoC2_p3	ATAGGACCCTAGAGGAAGAA	CATATTCGCCCTCTCCCTCT	45.0	55.0	53.6	58.4	126-243 bp	1.0	48.4
rpoC2_p4	ATAGGACCCTAGAGGAAGAA	TCATATTCGCCCTCTCCCTC	45.0	55.0	53.6	58.4	127-244 bp	1.0	48.3
rpoC2_p5	AGATATAGGACCCTAGAGGAAGA	ATATTCGCCCTCTCCCTCTC	43.5	55.0	56.2	58.1	129-246 bp	1.0	48.1