Hotspot

trnI-GAU-CD878_pgr001

IRa · 122548-122850 · 303 bp

Back to Heteropogon

Pi 0.0233

MarkerSeek score 61.30

Primer available yes

Label trnI-GAU-CD878_pgr001

Feature Metadata

label_name: trnI-GAU-CD878_pgr001
feature_type: igs
region: IRa
length_bp: 303
variable_sites: 13
pi: 0.0233
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-GAU-CD878_pgr001_p1	CTATCCATGTGGCTGAGAGC	TAAGGAGGGGGATGCCTAAG	55.0	55.0	57.8	57.9	478-479 bp	1.0	80.1
trnI-GAU-CD878_pgr001_p2	GGCACAACGACGCAATTATC	TAAGGAGGGGGATGCCTAAG	50.0	55.0	58.2	57.9	446-447 bp	1.0	80.0
trnI-GAU-CD878_pgr001_p3	CGCTGATGAGCACATTGAAC	TAAGGAGGGGGATGCCTAAG	50.0	55.0	57.8	57.9	497-498 bp	1.0	79.9
trnI-GAU-CD878_pgr001_p4	CTATCCATGTGGCTGAGAGC	GTAAGGAGGGGGATGCCTAA	55.0	55.0	57.8	58.2	479-480 bp	1.0	79.5
trnI-GAU-CD878_pgr001_p5	GATCCACATCTTCGGGTCAG	TAAGGAGGGGGATGCCTAAG	55.0	55.0	57.8	57.9	518-519 bp	1.0	79.4