Hotspot

ccsA-ndhD

SSC · 106692-106871 · 180 bp

Back to Heteropogon

Pi 0.0267
MarkerSeek score 65.10
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
180
variable_sites
11
pi
0.0267
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 GGTTCATTTACATTACCATCTAA AGGGTAGAGGTTCTATTATCCA 30.4 40.9 52.3 54.8 237-267 bp 1.0 46.4
ccsA-ndhD_p2 GGTTCATTTACATTACCATCTAAA AGGGTAGAGGTTCTATTATCCA 29.2 40.9 53.1 54.8 237-267 bp 1.0 46.4
ccsA-ndhD_p3 GGTTCATTTACATTACCATCTAA AGGGTAGAGGTTCTATTATCCAA 30.4 39.1 52.3 55.6 237-267 bp 1.0 46.4
ccsA-ndhD_p4 GGTTCATTTACATTACCATCTAA GTTGATAGGGTAGAGGTTCT 30.4 45.0 52.3 53.1 243-273 bp 1.0 46.3
ccsA-ndhD_p5 GGTTCATTTACATTACCATCTAAA GTTGATAGGGTAGAGGTTCT 29.2 45.0 53.1 53.1 243-273 bp 1.0 46.3