Hotspot

rpl32-trnL-UAG

SSC · 105038-105568 · 531 bp

Back to Heteropogon

Pi 0.0298
MarkerSeek score 65.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
531
variable_sites
33
pi
0.0298
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGAAGGATTGATTTGCGGCA TTCGAACCGAGATGCTTGAG 45.0 50.0 58.2 58.0 842-861 bp 1.0 82.9
rpl32-trnL-UAG_p2 AGAAGGATTGATTTGCGGCA GGATTCGAACCGAGATGCTT 45.0 50.0 58.2 58.1 845-864 bp 1.0 82.5
rpl32-trnL-UAG_p3 AGAAGGATTGATTTGCGGCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.2 57.9 815-834 bp 1.0 82.1
rpl32-trnL-UAG_p4 AGAAGGATTGATTTGCGGCA ATGTCCCGAAGAAACCCAAC 45.0 50.0 58.2 58.1 1128-1140 bp 1.0 81.8
rpl32-trnL-UAG_p5 AGAAGGATTGATTTGCGGCA GATCCGCACAAAAGAGTTGC 45.0 50.0 58.2 58.0 1394-1406 bp 1.0 81.7