Hotspot

CD878_pgr008-trnI-GAU

IRb · 93334-93636 · 303 bp

Back to Heteropogon

Pi 0.0233

MarkerSeek score 61.30

Primer available yes

Label CD878_pgr008-trnI-GAU

Feature Metadata

label_name: CD878_pgr008-trnI-GAU
feature_type: igs
region: IRb
length_bp: 303
variable_sites: 13
pi: 0.0233
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
CD878_pgr008-trnI-GAU_p1	TAAGGAGGGGGATGCCTAAG	CTATCCATGTGGCTGAGAGC	55.0	55.0	57.9	57.8	478-479 bp	1.0	80.1
CD878_pgr008-trnI-GAU_p2	TAAGGAGGGGGATGCCTAAG	GGCACAACGACGCAATTATC	55.0	50.0	57.9	58.2	446-447 bp	1.0	80.0
CD878_pgr008-trnI-GAU_p3	TAAGGAGGGGGATGCCTAAG	CGCTGATGAGCACATTGAAC	55.0	50.0	57.9	57.8	497-498 bp	1.0	79.9
CD878_pgr008-trnI-GAU_p4	GTAAGGAGGGGGATGCCTAA	CTATCCATGTGGCTGAGAGC	55.0	55.0	58.2	57.8	479-480 bp	1.0	79.5
CD878_pgr008-trnI-GAU_p5	TAAGGAGGGGGATGCCTAAG	GATCCACATCTTCGGGTCAG	55.0	55.0	57.9	57.8	518-519 bp	1.0	79.4