Hotspot

rpl16-rps3

LSC · 79817-79960 · 144 bp

Back to Heteropogon

Pi 0.0368
MarkerSeek score 65.60
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
144
variable_sites
10
pi
0.0368
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 AACGAGTCACACACTAAGCA TGCTGCTATCCAATTCGAACT 45.0 42.9 57.1 57.7 237-241 bp 1.0 66.1
rpl16-rps3_p2 ACGAGTCACACACTAAGCAT TGCTGCTATCCAATTCGAACT 45.0 42.9 56.9 57.7 236-240 bp 1.0 64.3
rpl16-rps3_p3 AACGAGTCACACACTAAGCAT TGCTGCTATCCAATTCGAACT 42.9 42.9 57.6 57.7 237-241 bp 1.0 62.1
rpl16-rps3_p4 AACGAGTCACACACTAAGCA TGCTGCTATCCAATTCGAAC 45.0 45.0 57.1 56.2 237-241 bp 1.0 62.0
rpl16-rps3_p5 ACGAGTCACACACTAAGCAT TGCTGCTATCCAATTCGAAC 45.0 45.0 56.9 56.2 236-240 bp 1.0 60.2