Hotspot

rbcL-psaI

LSC · 57042-57947 · 906 bp

Back to Heteropogon

Pi 0.0112

MarkerSeek score 58.90

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 906
variable_sites: 23
pi: 0.0112
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TGCAAATGGAGTGCTGAACT	AGGTACGAAAATAGAGGGTAAGT	45.0	39.1	58.0	56.5	1029-1088 bp	1.0	47.7
rbcL-psaI_p2	TGCAAATGGAGTGCTGAACT	AAGGTACGAAAATAGAGGGT	45.0	40.0	58.0	53.3	1030-1089 bp	1.0	46.7
rbcL-psaI_p3	GCCGCAGCTTGTGAAATATG	AGGTACGAAAATAGAGGGTAAGT	50.0	39.1	58.2	56.5	1008-1067 bp	1.0	46.5
rbcL-psaI_p4	TGCAAATGGAGTGCTGAACT	AAAGGTACGAAAATAGAGGGT	45.0	38.1	58.0	54.3	1031-1090 bp	1.0	46.0
rbcL-psaI_p5	GCCGCAGCTTGTGAAATATG	AAGGTACGAAAATAGAGGGT	50.0	40.0	58.2	53.3	1009-1068 bp	1.0	45.6