Hotspot

ndhC-trnV-UAC

LSC · 50945-51853 · 909 bp

Back to Heteropogon

Pi 0.00583
MarkerSeek score 65.20
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
909
variable_sites
12
pi
0.00583
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CTAACCGGGGCTAAAAGTCC GGTTCGAATCCGTATAGCCC 55.0 55.0 58.0 57.9 954-1071 bp 1.0 84.1
ndhC-trnV-UAC_p2 CTAACCGGGGCTAAAAGTCC AGCCCAATGCAGAAAAATGC 55.0 45.0 58.0 57.9 1047-1164 bp 1.0 83.8
ndhC-trnV-UAC_p3 CTAACCGGGGCTAAAAGTCC CGGTTCGAATCCGTATAGCC 55.0 55.0 58.0 58.3 955-1072 bp 1.0 82.2
ndhC-trnV-UAC_p4 TAACCGGGGCTAAAAGTCCT GGTTCGAATCCGTATAGCCC 50.0 55.0 58.3 57.9 953-1070 bp 1.0 81.6
ndhC-trnV-UAC_p5 TAACCGGGGCTAAAAGTCCT AGCCCAATGCAGAAAAATGC 50.0 45.0 58.3 57.9 1046-1163 bp 1.0 81.3