Hotspot

trnT-GGU-trnE-UUC

LSC · 15498-16028 · 531 bp

Back to Heteropogon

Pi 0.0181

MarkerSeek score 58.50

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 531
variable_sites: 22
pi: 0.0181
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	AGGCATAAGTCATCGGTTCA	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.6	58.3	564-621 bp	1.0	73.8
trnT-GGU-trnE-UUC_p2	AGGCATAAGTCATCGGTTCA	TGTCCTGAACCACTAGACGA	45.0	50.0	56.6	57.7	543-600 bp	1.0	73.8
trnT-GGU-trnE-UUC_p3	TGGTAAGGCATAAGTCATCGG	GCTGCCTCCTTGAAAGAGAG	47.6	55.0	57.5	58.3	569-626 bp	1.0	73.0
trnT-GGU-trnE-UUC_p4	GGTAAGGCATAAGTCATCGGT	GCTGCCTCCTTGAAAGAGAG	47.6	55.0	57.5	58.3	568-625 bp	1.0	73.0
trnT-GGU-trnE-UUC_p5	TGGTAAGGCATAAGTCATCGG	TGTCCTGAACCACTAGACGA	47.6	50.0	57.5	57.7	548-605 bp	1.0	73.0