Hotspot

trnG-UCC-trnT-GGU

LSC · 13769-15425 · 1657 bp

Back to Heteropogon

Pi 0.0111

MarkerSeek score 57.10

Primer available yes

Label trnG-UCC-trnT-GGU

Feature Metadata

label_name: trnG-UCC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1657
variable_sites: 38
pi: 0.0111
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnT-GGU_p1	TTTCCACCGAGCTGAAACAA	TCACTAGACGATAGCACCGT	45.0	50.0	58.0	58.0	879-2990 bp	1.0	85.4
trnG-UCC-trnT-GGU_p2	TTTTGCTTCGCGACTACGTA	TCACTAGACGATAGCACCGT	45.0	50.0	58.0	58.0	2514-2839 bp	1.0	85.2
trnG-UCC-trnT-GGU_p3	TTTTGCTTCGCGACTACGTA	GCATCGACCCAGGAAGAATT	45.0	50.0	58.0	58.0	2659-2984 bp	1.0	85.1
trnG-UCC-trnT-GGU_p4	TTTCCACCGAGCTGAAACAA	GCATCGACCCAGGAAGAATT	45.0	50.0	58.0	58.0	1024-2872 bp	0.6	65.4
trnG-UCC-trnT-GGU_p5	CATCGTTAGCTTGGAAGGCT	CACTCTGTGCTTCCTTCGTT	50.0	50.0	58.0	58.1	2667-2673 bp	0.4	54.8