Hotspot

ycf1

SSC · 125670-131171 · 5502 bp

Back to Heteroblemma

Pi 0.0141

MarkerSeek score 53.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5502
variable_sites: 77
pi: 0.0141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CTGTTTCGCCATTTGTGTGG	CCTTTTGGTTCTCCCCGAAA	50.0	50.0	58.2	58.0	986-995 bp	1.0	81.4
ycf1_p2	TTCGCCATTTGTGTGGTACT	CCTTTTGGTTCTCCCCGAAA	45.0	50.0	57.7	58.0	982-991 bp	1.0	81.1
ycf1_p3	CTGTTTCGCCATTTGTGTGG	CCTTCCTTTTGGTTCTCCCC	50.0	55.0	58.2	58.1	990-999 bp	1.0	80.9
ycf1_p4	ACGCTTGCGAAAAGTAGGAT	CCTTTTGGTTCTCCCCGAAA	45.0	50.0	57.9	58.0	745 bp	0.5	59.2
ycf1_p5	ACGCTTGCGAAAAGTAGGAT	CCTTCCTTTTGGTTCTCCCC	45.0	55.0	57.9	58.1	749 bp	0.5	58.6