Hotspot

ccsA-ndhD

SSC · 117133-117448 · 316 bp

Back to Heteroblemma

Pi 0.0447

MarkerSeek score 64.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 316
variable_sites: 14
pi: 0.0447
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTAGTACCCGCGCTTCAATC	TCAATGGCTTCCCTTGCATT	50.0	45.0	58.1	58.1	1213-1258 bp	1.0	83.4
ccsA-ndhD_p2	ATGCAGCTCTTTTATGCGGA	TCAATGGCTTCCCTTGCATT	45.0	45.0	58.0	58.1	1197 bp	0.5	59.2
ccsA-ndhD_p3	TGCAGCTCTTTTATGCGGAT	TCAATGGCTTCCCTTGCATT	45.0	45.0	58.0	58.1	1196 bp	0.5	59.2
ccsA-ndhD_p4	TGCAGCTCTTTTATGCGGAT	CTCGACAACATGGGTGGAAT	45.0	50.0	58.0	57.9	1259 bp	0.5	58.7
ccsA-ndhD_p5	TGCAGCTCTTTTATGCGGAT	TTTTGATTCTGGGCCGAGAG	45.0	50.0	58.0	57.9	978 bp	0.5	58.7