Hotspot

petA-psbJ

LSC · 63830-64732 · 903 bp

Back to Heteroblemma

Pi 0.0485

MarkerSeek score 78.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 903
variable_sites: 43
pi: 0.0485
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGTCTTTTGT	ATACTCAGGGTTGGGCTCAT	45.0	50.0	57.6	58.1	1043-1045 bp	1.0	80.4
petA-psbJ_p2	ACGTGTCCAAGGTCTTTTGT	GTTCATACTCAGGGTTGGGC	45.0	55.0	57.6	58.2	1047-1049 bp	1.0	79.2
petA-psbJ_p3	ACGTGTCCAAGGTCTTTTGT	TTCATACTCAGGGTTGGGCT	45.0	50.0	57.6	58.3	1046-1048 bp	1.0	78.6
petA-psbJ_p4	ACGTGTCCAAGGTCTTTTGT	GGTTCATACTCAGGGTTGGG	45.0	55.0	57.6	57.6	1048-1050 bp	1.0	77.8
petA-psbJ_p5	TGTTCTTCCTGGCATCTGTT	ATACTCAGGGTTGGGCTCAT	45.0	50.0	57.0	58.1	1026-1028 bp	1.0	76.1