Hotspot

accD-psaI

LSC · 59624-60490 · 867 bp

Back to Heteroblemma

Pi 0.0282

MarkerSeek score 65.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 867
variable_sites: 23
pi: 0.0282
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	ACAGTACCCGAGGATTCACA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	1025-1075 bp	1.0	85.1
accD-psaI_p2	TTGGAATGCTGGGCGATATC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	1114-1164 bp	1.0	84.4
accD-psaI_p3	GGTGACGGCTAGTTTTGGAA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	1128-1178 bp	1.0	84.2
accD-psaI_p4	GGAATGCTGGGCGATATCAT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.9	58.0	1112-1162 bp	1.0	84.2
accD-psaI_p5	CGATTGATCCAGGGACTTGG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1558-1608 bp	1.0	83.4