Hotspot

rps4-trnT-UGU

LSC · 46603-47016 · 414 bp

Back to Heteroblemma

Pi 0.0413

MarkerSeek score 73.30

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 414
variable_sites: 16
pi: 0.0413
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TAGACCTGGTAAAGCCCCAA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	540-541 bp	1.0	84.9
rps4-trnT-UGU_p2	TTAGACCTGGTAAAGCCCCA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.0	57.9	541-542 bp	1.0	84.9
rps4-trnT-UGU_p3	TGAAACGAGGCCCCCTATAA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	505-506 bp	1.0	84.5
rps4-trnT-UGU_p4	TTGAAACGAGGCCCCCTATA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.1	57.9	506-507 bp	1.0	84.5
rps4-trnT-UGU_p5	TAGACCTGGTAAAGCCCCAA	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.0	57.8	508-509 bp	1.0	84.2