Hotspot

trnC-GCA-petN

LSC · 28533-29377 · 845 bp

Back to Heteroblemma

Pi 0.0178

MarkerSeek score 68.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 845
variable_sites: 15
pi: 0.0178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.1	58.0	918-919 bp	1.0	80.7
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	TTTAAAGTCCACTTCGCCCC	50.0	50.0	58.1	58.1	975-976 bp	1.0	79.9
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	CAGCCCAAGCAAGACTTACT	50.0	50.0	58.1	57.8	912-913 bp	1.0	79.2
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	AGACGACCATTAAAGCAGCC	50.0	50.0	58.1	58.3	927-928 bp	1.0	78.6
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	TTAAAGCAGCCCAAGCAAGA	50.0	45.0	58.4	58.0	936-937 bp	1.0	78.5