Hotspot

atpH-atpI

LSC · 13790-14725 · 936 bp

Back to Heteroblemma

Pi 0.0272

MarkerSeek score 73.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 936
variable_sites: 25
pi: 0.0272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GAAGCAAGTCCGACAGCTAA	TAGCCGCCGCTTATATAGGA	50.0	50.0	57.9	57.9	1042-1048 bp	1.0	81.4
atpH-atpI_p2	GAAGCAAGTCCGACAGCTAA	TTTTTGCAACTTTAGCCGCC	50.0	45.0	57.9	58.1	1054-1060 bp	1.0	81.3
atpH-atpI_p3	AACAGAAGCGGCAGAAATCA	TAGCCGCCGCTTATATAGGA	45.0	50.0	58.1	57.9	1010-1016 bp	1.0	81.3
atpH-atpI_p4	AACAGAAGCGGCAGAAATCA	TTTTTGCAACTTTAGCCGCC	45.0	45.0	58.1	58.1	1022-1028 bp	1.0	81.2
atpH-atpI_p5	CAGCAGCAATAACAGAAGCG	TAGCCGCCGCTTATATAGGA	50.0	50.0	57.8	57.9	1020-1026 bp	1.0	80.8