Hotspot

trnH-GUG-psbA

LSC · 76-299 · 224 bp

Back to Heteroblemma

Pi 0.0452

MarkerSeek score 79.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 224
variable_sites: 10
pi: 0.0452
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.1	57.9	291-292 bp	1.0	82.0
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	341-342 bp	1.0	81.8
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	355-356 bp	1.0	81.6
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	354-355 bp	1.0	81.6
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	356-357 bp	1.0	81.6