Hotspot

rps15-ycf1

SSC · 129645-130047 · 403 bp

Back to Heteranthera

Pi 0.0431

MarkerSeek score 81.40

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 403
variable_sites: 62
pi: 0.0431
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCTTCTTTTTCTTCTTGTGGA	TGGTAGTCGTTTCAGTATGT	33.3	40.0	52.9	53.8	497-577 bp	1.0	52.0
rps15-ycf1_p2	TGTTTTCTTCTTTTTCTTCTTGTGGA	TGGTAGTCGTTTCAGTATGT	30.8	40.0	58.1	53.8	502-582 bp	1.0	52.0
rps15-ycf1_p3	TGTTTTCTTCTTTTTCTTCTTGTGG	TGGTAGTCGTTTCAGTATGT	32.0	40.0	56.9	53.8	502-582 bp	1.0	52.0
rps15-ycf1_p4	CTGTTTTCTTCTTTTTCTTCTTGTGG	TGGTAGTCGTTTCAGTATGT	34.6	40.0	57.8	53.8	503-583 bp	1.0	52.0
rps15-ycf1_p5	TCTTCTTTTTCTTCTTGTGGAA	TGGTAGTCGTTTCAGTATGT	31.8	40.0	53.8	53.8	497-577 bp	1.0	52.0