Hotspot

rbcL-accD

LSC · 60712-61470 · 759 bp

Back to Heteranthera

Pi 0.0371

MarkerSeek score 82.70

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 759
variable_sites: 102
pi: 0.0371
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GGCTGCTTGTGAAGTATGGA	TAACTTCCCGTTGGACAACC	50.0	50.0	57.9	57.7	871-935 bp	1.0	89.0
rbcL-accD_p2	CCGTGAAGCTACCAAATGGA	TAACTTCCCGTTGGACAACC	50.0	50.0	57.9	57.7	904-968 bp	1.0	88.9
rbcL-accD_p3	TCCGTGAAGCTACCAAATGG	TAACTTCCCGTTGGACAACC	50.0	50.0	57.9	57.7	905-969 bp	1.0	88.9
rbcL-accD_p4	CCAAATGGAGCCCTGAACTT	TAACTTCCCGTTGGACAACC	50.0	50.0	58.1	57.7	893-957 bp	0.909091	85.0
rbcL-accD_p5	CCAAATGGAGCCCTGAACTT	GCTCTAACTTCCCGTTGGAC	50.0	55.0	58.1	58.3	897-961 bp	0.909091	84.9