Pi
0.0422
MarkerSeek score
89.70
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | AGAGAAAAAGCCGGCTATCG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 1126-1223 bp | 1.0 | 91.5 |
| trnT-UGU-trnL-UAA_p2 | CGATAGAGAAAAAGCCGGCT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 1130-1227 bp | 1.0 | 91.5 |
| trnT-UGU-trnL-UAA_p3 | TCGATAGAGAAAAAGCCGGC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 1131-1228 bp | 1.0 | 91.5 |
| trnT-UGU-trnL-UAA_p4 | AGAGAAAAAGCCGGCTATCG | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.1 | 58.1 | 1134-1231 bp | 1.0 | 91.0 |
| trnT-UGU-trnL-UAA_p5 | CGATAGAGAAAAAGCCGGCT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.1 | 58.1 | 1138-1235 bp | 1.0 | 91.0 |