Hotspot

trnS-GGA-rps4

LSC · 47648-47939 · 292 bp

Back to Heteranthera

Pi 0.0327

MarkerSeek score 79.80

Primer available yes

Label trnS-GGA-rps4

Feature Metadata

label_name: trnS-GGA-rps4
feature_type: igs
region: LSC
length_bp: 292
variable_sites: 39
pi: 0.0327
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-rps4_p1	CCGAGTGGTTGAAGGTGTAG	ATTATTCTCGCCAGACTTGA	55.0	40.0	57.9	54.2	388-394 bp	1.0	59.0
trnS-GGA-rps4_p2	AGGTGTAGCATTGGAACTGC	ATTATTCTCGCCAGACTTGA	50.0	40.0	58.2	54.2	376-382 bp	1.0	58.3
trnS-GGA-rps4_p3	TAGGCTTTTGCTTACCGAGG	ATTATTCTCGCCAGACTTGA	50.0	40.0	57.6	54.2	352-358 bp	1.0	56.7
trnS-GGA-rps4_p4	ATGTAGGAGAGATGGCCGAG	ATTATTCTCGCCAGACTTGA	55.0	40.0	58.4	54.2	403-409 bp	1.0	56.4
trnS-GGA-rps4_p5	TTTTGCTTACCGAGGGTTCG	ATTATTCTCGCCAGACTTGA	50.0	40.0	58.5	54.2	347-353 bp	1.0	56.0