Hotspot

rpoB-trnC-GCA

LSC · 28759-29952 · 1194 bp

Back to Heteranthera

Pi 0.0229

MarkerSeek score 76.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1194
variable_sites: 107
pi: 0.0229
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTTCCATCATTCCGGAGCA	GCGACACCCAGATTTGAACT	45.0	50.0	57.3	58.5	1276-1318 bp	0.909091	76.1
rpoB-trnC-GCA_p2	TTTCCATCATTCCGGAGCAT	GCGACACCCAGATTTGAACT	45.0	50.0	57.3	58.5	1275-1317 bp	0.909091	76.1
rpoB-trnC-GCA_p3	TTTCCATCATTCCGGAGCAT	CCTTACCACTTGGCCATGTC	45.0	55.0	57.3	58.5	1229-1271 bp	0.909091	76.0
rpoB-trnC-GCA_p4	ATTTCCATCATTCCGGAGCA	TAAAGGATTTGCAGTCCCCC	45.0	50.0	57.3	57.5	1251-1293 bp	0.909091	75.9
rpoB-trnC-GCA_p5	TTTCCATCATTCCGGAGCAT	TAAAGGATTTGCAGTCCCCC	45.0	50.0	57.3	57.5	1250-1292 bp	0.909091	75.9