Hotspot

rpoC2-rpoC1

LSC · 22497-22693 · 197 bp

Back to Heteranthera

Pi 0.0487

MarkerSeek score 78.60

Primer available yes

Label rpoC2-rpoC1

Feature Metadata

label_name: rpoC2-rpoC1
feature_type: igs
region: LSC
length_bp: 197
variable_sites: 34
pi: 0.0487
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rpoC1_p1	TTGTGAAAGACCAGATCGGC	AGAGGAAGCCGTACAAGGAT	50.0	50.0	58.2	58.1	272-285 bp	1.0	89.4
rpoC2-rpoC1_p2	GTGAAAGACCAGATCGGCTC	AGAGGAAGCCGTACAAGGAT	55.0	50.0	58.4	58.1	270-283 bp	1.0	88.1
rpoC2-rpoC1_p3	TTGTGAAAGACCAGATCGGC	TAGAGGAAGCCGTACAAGGA	50.0	50.0	58.2	57.5	273-286 bp	1.0	86.3
rpoC2-rpoC1_p4	TTGTGAAAGACCAGATCGGC	GCCGTACAAGGATTTTGTCG	50.0	50.0	58.2	57.5	265-278 bp	1.0	86.3
rpoC2-rpoC1_p5	TTGTGAAAGACCAGATCGGC	TCGAGCCTACTCATACGCTA	50.0	50.0	58.2	57.5	248-261 bp	0.909091	82.5