Hotspot

atpH-atpI

LSC · 14931-16439 · 1509 bp

Back to Heteranthera

Pi 0.0329

MarkerSeek score 78.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1509
variable_sites: 170
pi: 0.0329
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACGGAAGCAGCAGAAATCA	TAGGTGAATCCATGGAGGGT	45.0	50.0	58.1	57.4	1493-1629 bp	0.909091	82.8
atpH-atpI_p2	GCCAATCCAGCAGCAATAAC	TAGGTGAATCCATGGAGGGT	50.0	50.0	57.8	57.4	1510-1646 bp	0.909091	81.9
atpH-atpI_p3	TCCAGCAGCAATAACGGAAG	TAGGTGAATCCATGGAGGGT	50.0	50.0	58.3	57.4	1505-1641 bp	0.909091	81.6
atpH-atpI_p4	AACGGAAGCAGCAGAAATCA	AGGTGAATCCATGGAGGGTC	45.0	55.0	58.1	58.8	1492-1628 bp	0.909091	81.3
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	AGGTGAATCCATGGAGGGTC	50.0	55.0	57.8	58.8	1509-1645 bp	0.909091	80.4