Hotspot

ycf2-trnI-CAU

IRa · 149903-149970 · 68 bp

Back to Hetaeria

Pi 0.049

MarkerSeek score 70.00

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 68
variable_sites: 5
pi: 0.049
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	TTGTTGCACCCTGTGAATCA	GTTGGGCGCTTTAACCATTC	45.0	50.0	57.9	58.0	311-483 bp	1.0	80.8
ycf2-trnI-CAU_p2	AATCATGGTCCTTGCGGATC	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	797-969 bp	1.0	80.5
ycf2-trnI-CAU_p3	AATCATGGTCCTTGCGGATC	CACTTGACTGTCTACGGCTC	50.0	55.0	58.0	58.0	1370-1542 bp	1.0	80.4
ycf2-trnI-CAU_p4	CGAACCCCGGTTAGATTCAG	GTTGGGCGCTTTAACCATTC	55.0	50.0	58.1	58.0	634-806 bp	1.0	80.4
ycf2-trnI-CAU_p5	AATCATGGTCCTTGCGGATC	CCACAACGATGCTGTCCATA	50.0	50.0	58.0	58.0	1417-1589 bp	1.0	80.3