Hotspot

ndhI-ndhA

SSC · 117743-117855 · 113 bp

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Pi 0.0944

MarkerSeek score 73.30

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 113
variable_sites: 16
pi: 0.0944
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	TCCAATGTACCTTGCAGCTC	ACCAGAAGCAGAGGAGGAAT	50.0	50.0	57.9	58.0	576-590 bp	1.0	81.8
ndhI-ndhA_p2	ACGAAAACGCTCTGATGTGA	ACCAGAAGCAGAGGAGGAAT	45.0	50.0	57.9	58.0	669-683 bp	1.0	81.3
ndhI-ndhA_p3	ACGCGAACACATACTTCACA	ACCAGAAGCAGAGGAGGAAT	45.0	50.0	57.9	58.0	725-739 bp	1.0	81.1
ndhI-ndhA_p4	TCCAATGTACCTTGCAGCTC	GCCCATAGAGAAGCCGTATG	50.0	55.0	57.9	57.9	807-821 bp	1.0	80.9
ndhI-ndhA_p5	TCCAATGTACCTTGCAGCTC	TAGGCTTCATGTGCGGTTTT	50.0	45.0	57.9	58.1	782-796 bp	1.0	80.8