Hotspot

ccsA-ndhD

SSC · 113583-113856 · 274 bp

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Pi 0.0487

MarkerSeek score 70.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 274
variable_sites: 19
pi: 0.0487
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTTTACCCAAGGTTTCGCCA	TTTTTGATTCTGGGCCACGA	45.0	45.0	57.9	58.0	1059-1087 bp	1.0	84.4
ccsA-ndhD_p2	TTTTACCCAAGGTTTCGCCA	TTCTCGATGGCTTCTCTTGC	45.0	50.0	57.9	58.0	1279-1307 bp	1.0	83.9
ccsA-ndhD_p3	TTTTACCCAAGGTTTCGCCA	CTCGATGGCTTCTCTTGCAT	45.0	50.0	57.9	58.0	1277-1305 bp	1.0	83.7
ccsA-ndhD_p4	GCATGGGGCTCTTATTGGAA	TTTTTGATTCTGGGCCACGA	50.0	45.0	57.9	58.0	647-654 bp	1.0	83.3
ccsA-ndhD_p5	GCATGGGGCTCTTATTGGAA	TTCTCGATGGCTTCTCTTGC	50.0	50.0	57.9	58.0	867-874 bp	1.0	82.9